Invitrogen lipofectamine 2000 manual

Lipofectamine ™ Reagents Go online to view related products. ∤. Lipofectamine ™ Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells and especially designed for hard to transfect cells. Important Guidelines. ∤. Make DNA-Lipofectamine ™ complexes in serum-free medium such. DNA-Lipofectamine CD complexes can be added directly to cells in culture medium. It is not necessary to remove complexes or change/add medium after transfection, but complexes may be removed after 4–6 hours. PCR and Real-Time PCR, Reverse Transcription, cDNA Synthesis Direct from Cells. Researchers use Lipofectamine Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies. Lipofectamine Transfection Reagent is best transfection reagent available from Life Technologies for the co-transfection of siRNA and plasmid DNA. Features: 1.

• Lipofectamine™ 试剂用量各有不同。测试推荐的两 种浓度的Lipofectamine™ 试剂，以确定最佳用量，开 始新的转染。 在线资源 请登录我们的产品页面，了解更多信息和实验 方案。 Lipofectamine™ 试剂 用户指南. Invitrogen™ Lipofectamine™ Transfection Reagent is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells providing the following advantages: Highest transfection efficiency in many cell types and formats (e.g. well). The trusted, simple, broad-spectrum transfection reagent for most cell lines. Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal www.doorway.ruogen Lipofectamine Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect a variety of payloads into the widest variety of adherent.

The trusted, simple, broad-spectrum transfection reagent for most cell lines. Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Invitrogen Lipofectamine Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect a variety of payloads into the widest variety of adherent and suspension cell lines. Lipofectamine® DNA Transfection Reagent Protocol. Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (well), duplicate (well), and single well (6-well) transfections, and accounts for pipetting variations. Lipofectamine reagent outperforms Invitrogen Lipofectamine and FuGENE HD reagents. Each reagent was used to transfect HEK , HeLa, LNCaP, HepG2, and A cell lines in a well format, and GFP expression was analyzed 48 hours posttransfection. Lipofectamine reagent provided higher GFP transfection efficiency than Lipofectamine and FuGENE HD reagents for all five cell lines.